The spatial variability is normal and frequent occurence in vineyards which has been always known to growers. It is manifested in vine vigour, yield and quality of grapes, and it is most affected by differences in vineyards relief and soil and slightly less affected by diffrences in the position of vines within vineyard. As a consequence, different styles of wine can be produced from different parts of the vineyard. The variability of the vine vigour is associated with changes in physical and chemical properties of the soil and affects the yield and quality of grapes and wine. In this research, the spatial variability is analyzed in vineyards of variety 'Kraljevina', in winegrowing subregion Prigorje-Bilogora, in western continental Croatia. Variations in the quality of grapes (must) are shown by data on sugar content and titratable acidity obtained by chemical methods. According to the above data, it can be concluded that titratable acidity in the must of experimental vines was mainly elevated, while, on the other hand, the values of sugar content were slightly lower. The average number of clusters and the average weight of clusters per vine affect the total yield per vine, therefore, these parameters were of great importance for the description variations in yield in observed vineyards. The vigour of the vine is described by using the Ravaz index and the nitrogen content of the leaf dry matter. This index ia an excellent indicator of the relationship between the generative and vegetative potential of the vine. More than half of the vines in observed vineyards have values of the Ravaz index higher or lower than the optimal range which indicated that vines were either with lower vigour or higher vigour. Imbalance in vines, whether manifested in high or extremly low vigour, has a negative impact on overall growth and development of the vine so in that situations it is necessery to apply certain partices such as grater intensity prunning for the high vine vigour or nitrogen fertilizers for those with the low vigour.