This thesis features the synthesis of a dipeptide and a tripeptide in solution using the Boc-protecting group chemistry. The target molecules were Boc-Arg(2Z)-Arg(2Z)-Tyr[m-CH2N(CH3)2]-OBn (1) and BODIPY-Arg(2Z)-Arg(2Z)-OBn (2). Tripeptide 1 contains a photochemically reactive modified tyrosine, which can generate quinone methide in the photochemical reaction, while dipeptide 2 contains a BODIPY fluorophore. Both peptides contain a diarginine fragment for which non-covalent bonding to polynucleotides is anticipated. Furthermore, upon the photochemical excitation of the compound 1 non-covalently bound to a polynucleotide, the generation of quinone methides is anticipated, leading to the alkylation of polynucleotides, whereas the fluorophore in compound 2 was introduced for the visualization of the non-covalent binding to develop fluorescent indicators for polynucleotides. We successfully synthesized Boc-Tyr-OH (4, η = 88 %), Boc-Tyr-OBn (5, η = 65 %), Boc-Tyr[m-CH2N(CH3)2]-OBn (6, η = 69 %), Boc-Arg(2Z)-OH (9, η = 49 %), Boc-Arg(2Z)-OMe (11, η = 25 %), Boc-Arg(2Z)-Arg-(2Z)-OMe (14, η = 66 %), Boc-Arg(2Z)-ONHS (16, η = 68 %), Boc-Arg(2Z)-Arg(2Z)-OH (15, η = 27 %), Boc-Arg(2Z)-OBn (18, η = 98 %), Boc-Arg(2Z)-Arg(2Z)-OBn (20, η = 58 %), BODIPY-Arg(2Z)-Arg(2Z)-OBn (2, η = 32 %), while Boc-Arg(2Z)-Arg(2Z)-Tyr[m-CH2N(CH3)2]-OBn (1, η = 9 %) was obtained in a mixture with other compounds. All synthesized compounds were characterized by 1H and 13C NMR spectroscopy. Additionally, compound 2 was characterized by high-resolution mass spectrometry, FTIR-ATR and melting point determination. To investigate photophysical properties, we conducted UV-Vis and fluorescence measurements. For compound 2 in acetonitrile solution, we determined the molar absorption coefficient (ε = 90500 dm^3 mol^-1 cm^-1 at 499 nm), fluorescence quantum yield (ՓF = 0,52) and lifetime of the singlet excited state (τ = 1,21 ns).