Research goal: Test the level of knowledge regarding information safety and privacy among the nurses and technicians employed at the Clinical Hospital Centre Osijek, considering their gender and completed education, and test the level of potential risk resulting from their behaviour online, when compared to the previous study. Study plan: The conducted study was implemented as a cross-sectional study. Research participants and methods: 90 nurses and technicians employed at the Clinical Hospital Centre Osijek participated in the study, their level of completed education was secondary school, college, and higher education. The validated scientific questionnaire “Questionnaire on the Knowledge and Risky Behaviour of the Information System Users” was used as a research instrument. The questionnaire contains 33 questions evaluated using points on the Likert scale of 1 to 5, where the offered responses have different meanings. Two control questions were added at the end of the survey questionnaire. A survey questionnaire containing demographic information and 7 questions was used. Results: There is significant difference in two subscales, when compared to the previous study, first one is about personal computer maintenance, and the second one is about secured data, in which is significant difference regarding gender. Also, there is significant difference in first subscale regarding completed education and in third one regarding age. Conclusion: The nurses and technicians employed at the Clinical Hospital Centre Osijek do not differ from the average concerning their knowledge, when compared to the previous study. In one segment they are even above the average, but their behaviour online is potentially risky.