Zinc is essential for the growth, development and proper functioning of the human body. Zinc plays an important role in cell proliferation, differentiation and metabolic activity of the cell. Any all change the zinc concentrations in the body can lead to the development and progression of many diseases (eg. Acrodermatitis enteropathy). Furthermore, it is extremely important to regulate the status of zinc in the body. In the case of zinc defficiency in human body it is necessary to adjust the diet or supplements to compensate for its deficiency. There are various pharmaceutical forms and methods of zinc intake on the market, but the most commonly used route is tablet or capsule delivery. The levels of zinc increases in the case of acute or chronic poisoning with zinc in humans is rare. The aim of this research work was to validate AAS method and to apply it precisely and reliably to determine the concentration of zinc in selected samples of supplements. Furthermore, by comparing the measured data with those provided in the sample composition declarations, it was necessary to ascertain whether the test complies with the requirements oft he declared standards. Sample analysis carried out by FAAS analytical method. Four samples of dietary supplements were collected from various manufactures during 2017. Zinc from the sample blend tables was quantitatively extracted to the protocol and a primary solution was obtained. Dilution oft he primary solution (1:1000) provided solutions that were subjected to the assay. Every day, prior to sample measurement, the method was validated and showed satisfactory validation parameter values. After a three-day analysis, the result were processed and the values of zinc mass were obtained in the samples of supplements. Comparison of the same with the zinc masses mentioned on the product declarations has been shown to be minor deviations that can be attributed to systematic and random. In addition, it is possible that the content of zinc, in the selected samples, which were subjected to the analyzes, deviations from the masses indicated on the product declarations.